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Catalogue: GRDC Final Reports
Chickpea itself has no close wild relatives within its species, Cicer arietinum , and earlier attempts to cross chickpea with distant relatives using conventional hybridisation methods were unsuccessful... We conclude that barriers to wide crosses in chickpea using conventional methods occur after fertilisation, leading to abortion of the hybrid seed before maturity... The success of the project in achieving confirmed hybrids of chickpea X C. bijugum in tissue culture, which have never been achieved using conventional methods, has created much interest among chickpea breeders and researchers and we have been encouraged to continue the research...
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Catalogue: GRDC Final Reports
Wild relatives of chickpea among the genus Cicer have many valuable agronomic traits crucial for improvement of the cultivar... Methods developed for wide crosses with C. pinnatifidum at ICRISAT, India (Mallikarjuna, 1999) were transferred and adapted for crosses between local chickpea cultivars, in Canada and Australia, and C. pinnatifidum... Embryo rescue has good potential as the key to unlocking the treasure chest of distant wild relatives for chickpea improvement...
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Catalogue: GRDC Final Reports
Chickpea itself has no close wild relatives within its species Cicer arietinum, and earlier attempts to cross chickpea with distant relatives using conventional hybridisation methods were unsuccessful... The results of experimental research are used to improve the process as tissue culture media and methods continue to be optimised in ongoing research... 9) One week later, or when plantlets have grown about the way up the vessel, remove the culture vessel completely 10) Cover the plantlet with a clear cellophane bag with aeration holes (we use supermarket bread bags)...
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Catalogue: GRDC Final Reports
Embryo rescue and plant regeneration in vitro of selfed chickpea (Cicer arietinum L.) and it's wild annual relatives.. The aims of this study were to examine embryogenesis in selfed and hybridised ovules; to develop robust techniques to rescue immature embryos in_vitro using selfed chickpea ovules; to regenerate plantlets from rescued chickpea ovules; and to apply the best techniques to rescue interspecific hybrids... Some cross pollinated ovules were rescued in_vitro and putative hybrid plantlets were regenerated...
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