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dry bulk seed test

Catalogue: GRDC Final Reports
Use of the test may also help to prevent wheat breeders sowing infected seed stocks... Use of the test confirmed widespread occurrence of WSMV in the WA grainbelt and identified the disease for the first time in Tasmania... The research also showed that Australian WSMV isolates represent a single incursion from overseas and the virus has been present in Australia for at least 15 years...
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Catalogue: GRDC Final Reports
" Attachment 1 Project DAW00141 Developing a sensitive dry seed test to detect seed-borne viruses in bulk seed samples of wheat Procedure for RT-PCR detection of WSMV in bulk seed samples of wheat [Procedure developed for detection of Wheat streak mosaic virus (WSMV) in dry, bulk seed samples of wheat seed during GRDC project DAW 00141 "Developing a sensitive dry seed test to detect seed-borne viruses in bulk seed samples of wheat"... The test does not provide any quantitative indication of the precise level of WSMV seed transmission to seedlings in the seed stock tested which may be crucial information for growers when seed is to be sown on farms with a previous history of WSMV in their crops... The dry, bulk seed test is based on RT-PCR so its extremely high sensitivity means that great caution must be taken to avoid contamination of seed with dust that may carry wheat micro-trash contaminated with WSMV...
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