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The hypothesis for our studies is that optical and transmission electron microscopy (TEM) studies of embryogenesis in interspecific hybrids will aid in the identification of such barriers... For hybridisation, buds of chickpea were emasculated before anthesis, and the stigma was hand pollinated with fresh pollen from C. bijugum to create the interspecific hybrids... In summary, optical microscopy studies of embryogenesis, in both selfs and hybrids, identified deleterious changes in the fertilised hybrid seed as early as 2 to 4 days after pollination in some crosses...
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