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A molecular approach to unravel the dynamics of disease suppressive microbial communities

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We propose to use a polyphasic approach involving (i) comparative analysis of high- and low-suppressive soils, (ii) pre-treatment or prior exposure of soils to factors that have been attributed to the development of disease suppression and (iii) amplification of target regions prior to high-throughput sequencing... The use of direct soil DNA/RNA extraction technologies will help overcome the bottlenecks associated with the culture based methods... We propose to establish close working links with other projects in SBI-Theme 3 for field sites used, consistency in methodologies and complementary research approaches...
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