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Manipulating biological processes that improve nitrogen supply to cereal crops: Free-living nitrogen fixing bacteria

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Activity 1: Estimation of the actual amount of N fixed: In Australia, most estimates of FL-N fixation were determined using indirect methods, e.g. N balance estimates or nitrogenase activity measurements using acetylene reduction assay... While the estimates provide an indication of N fixation potential, they do not provide direct estimates of N fixed... Estimation of the actual amount of N fixed quantify the amount of N fixed - Acombination of 15N isotope tracer methodology and biochemical methods are being standardized to quanity the amount of 15N fixed using soils from South Australia (Calcorosol, Alfisol, Vertisol) and Western Australia (Sodosol)...